COL2A1 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, FC, IF, IHC, IP, WB |
|---|---|
| Recommended Dilution | WB: User Optimized IHC: 1:200 - 1:1,000 IF: User Optimized FC: User Optimized ELISA: 1:10,000 - 1:50,000 IP: 1:100 |
| Reactivity | Bovine, Human |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Anti-Collagen Type II antibody was produced by repeated immunizations with Collagen Type II from adult human knee cartilage and bovine nasal cartilage. |
| Specificity | This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other collagens. Some class specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type II collagens and has expected cross-reactivity with Type IV and VI and negligible cross reactivity to Type I, III and V collagens. Non-specific cross reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. |
| Buffer | 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Reconstitution Method | Restore with deionized water (or equivalent) - Reconstitution Volume: 50µL |
| Concentration | 1.0 mg/ml - lot specific |
| Conjugation | FITC |
| Storage | Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Ambient |
| Gene Name | collagen type II alpha 1 chain |
| Database Link | |
| Background | Anti-Collagen Type II Fluorescein Conjugated Antibody detects Type-II collagen which is the basis for articular cartilage and hyaline cartilage. It makes up 50% of all protein in cartilage and 85-90% of collagen of articular cartilage. Type II collagen does form fibrils which allow cartilage to entrap the proteoglycan aggregate as well as provide tensile strength to the tissue. Anti-Collagen Type II antibody is ideal for investigators involved in Immunology, Neuroscience and Signal Transduction research. |
| Synonyms | rabbit anti-Collagen Type II antibody fluorescein conjugation, FITC conjugated rabbit anti-Collagen Type II antibody, Collagen alpha-1 (II) chain, Alpha-1 type II collagen, Cartilage collagen antibody, Chondrocalcin antibody, COL2A1 antibody |
| Note | Anti-Collagen Type II Fluorescein Conjugated Antibody is suitable for western blotting, IP, IHC and for ELISA. Researchers should determine optimal titers for applications that are not stated below. |
| Reference Data | |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.