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LPO Sheep Polyclonal Antibody

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SKU
TA397082S
Anti-Lactoperoxidase (Bovine Milk) (SHEEP) Antibody, 25 uL
Size:
25 ul
Conjugation:
Unconjugated
  $300.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Application ELISA, IP, WB
Recommended Dilution WB: 1:500 - 1:5,000
ELISA: 1:5,000 - 1:20,000
IP: 1:100
Reactivity Bovine
Antibody Host Sheep
Clonality Polyclonal
Immunogen Lactoperoxidase Bovine Milk
Specificity Anti-LACTOPEROXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Lactoperoxidase Bovine Milk.  Cross reactivity against Lactoperoxidase from other tissues and species may occur but have not been specifically determined.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01% (w/v) Sodium Azide
Concentration 1 mg/mL - lot specific
Conjugation Unconjugated
Storage Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Database Link
Background Lactoperoxidase is an antimicrobial agent which utilizes hydrogen peroxide and thiocyanate (SCN) to generate the antimicrobial substance hypothiocyanous acid (HOSCN). It may protect the udder from infection and promote growth in newborn calves. It inhibits growth of the following bacterial species: E.coli, K.pneumoniae, P.aeruginosa, S.sonnei, S.saphrophyticus, S.epidermidis, and S.dysenteriae.
Synonyms sheep anti-Lactoperoxidase Antibody, LPO antibody, Salivary peroxidase antibody, SAPX antibody, SPO antibody
Note Lactoperoxidase antibody has been tested by western blot and is suitable to be assayed against 1.0 ug of Lactoperoxidase Bovine Milk in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG L - Cat# H (Rabbit) code #613-4302 and (ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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