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PGA Goat Polyclonal Antibody

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SKU
TA396729S
Anti-Pepsin (GOAT) Antibody Peroxidase Conjugated, 25 uL
Size:
25 ul
Conjugation:
HRP
  $300.00
5 Days*
Bulk/Customize

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Specifications
Specifications
Product Data
Application ELISA, IHC, WB
Recommended Dilution WB: 1:500 - 1:2,500
IHC: 1:250 - 1:1,250
ELISA: 1:2,000 - 1:10,000
Reactivity Pig
Antibody Host Goat
Clonality Polyclonal
Immunogen Pepsin Porcine Stomach
Specificity Pepsin is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pepsin Porcine Stomach.  Cross reactivity against Pepsin from other tissues and species may occur but have not been specifically determined.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Concentration 1.0 mg/mL - lot specific
Conjugation HRP
Storage Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Database Link
Background Pepsin is one of three principal protein-degrading, or proteolytic, enzymes in the digestive system, the other two being chymotrypsin and trypsin. Pepsin is commonly used in the preparation produce either an Fab or an F(ab')2 fragment from antibodies.
Synonyms goat anti-Pepsin Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Pepsin Antibody, Pepsin A antibody, Pepsin a precursor antibody, Pepsin antibody, Pepsinogen 1 second locus antibody, Pepsinogen 3 group 1 antibody, Pepsinogen a3 antibody
Note Anti-Pepsin peroxidase has been tested by ELISA, dot blot, and western blot and is useful in immunohistochemistry. Optimal titers should be obtained by researchers if not provided.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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