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NAM-DH Goat Polyclonal Antibody

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SKU
TA396697S
Anti-N-Acylmannosamine-1-Dehydrogenase (Pseudomonas) (GOAT) Antibody Biotin Conjugated, 25 uL
Size:
25 ul
Conjugation:
Unconjugated
  $300.00
5 Days*
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Specifications
Specifications
Product Data
Application ELISA, IP, WB
Recommended Dilution WB: 1:250 - 1:1,000
ELISA: 1:1,000 - 1:5,000
IP: 1:100
Reactivity Bacteria
Antibody Host Goat
Clonality Polyclonal
Immunogen N-Acyl Mannosamine-1-Dehydrogenase Recombinant Sequence from Pseudomonas expressed in E.coli
Specificity Anti-N-Acylmannosamine-1-Dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified N-Acylmannoseamine-1-Dehydrogenase Recombinant from Pseudomonas.  Cross reactivity against N-Acylmannoseamine-1-Dehydrogenase from other sources is unknown.
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01% (w/v) Sodium Azide
Concentration 1.0 mg/mL - lot specific
Conjugation Unconjugated
Storage Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Stability Expiration date is one (1) year from date of receipt.
Shipping Blue Ice
Database Link
Background Anti-N-Acylmannosamine-1-Dehydrogenase recognizes the protein N-Acylmannosamine 1-Dehydrogenase, a member of the oxidoreductase family that act on CH-OH donors and NAD+ or NADP+ as acceptors. N-acyl-D-mannosamine 1-dehydrogenase catalyzes the reaction in which N-acyl-D-mannosamine and NAD+ are converted to N-acyl-D-mannosaminolactone, NADH, and H+.
Synonyms goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody biotin Conjugation, Biotin Conjugated goat anti-N-Acylmannosamine-1-Dehydrogenase Antibody, N-acylmannosamine 1-dehydrogenase, NAM-DH
Note Anti-N-Acylmannosamine-1-Dehydrogenase antibody has been tested by western blot and is suitable to be assayed against 1.0 µg of N-Acylmannoseamine-1-Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:7,000 to 1:35,000 of the reconstitution concentration is suggested for this product.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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