gdhB Goat Polyclonal Antibody
$300.00
5 Days*
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:3,000 ELISA: 1:5,000 - 1:25,000 |
| Reactivity | Bacillus sp. |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Glucose Dehydrogenase Bacillus species |
| Specificity | Anti-Glucose Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucose Dehydrogenase Bacillus species. Cross reactivity against Glucose Dehydrogenase from other sources is unknown. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Database Link | |
| Background | Anti-Glucose Dehydrogenase antibody recognizes glucose dehydrogenase. A member of the oxidoreductase family that acts on CH-OH group on donors, glucose dehydrogenase uses NAD+ or NADP+ as the acceptor. Glucose dehydrogenase reacts with beta-D-glucose to produce D-glucono-1,5-lactone and depending on the acceptor, either NADH or NADPH. Anti-Glucose Dehydrogenase is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction. |
| Synonyms | goat anti-Glucose Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Glucose Dehydrogenase Antibody, gdh antibody, gdh2 antibody, gdh3 antibody, gdh4 antibody, gdhI antibody, GLCDHIII antibody, GLCDHIV antibody, Glucose 1 dehydrogenase 1 antibody |
| Note | Anti-Glucose Dehydrogenase has been assayed against 1.0 ug of Glucose Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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