SUC1 Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:2,500 ELISA: 1:1,000 - 1:5,000 |
| Reactivity | Candida sp |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Invertase Candida |
| Specificity | Anti-Invertase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Invertase Candida. Cross reactivity against Invertase from other sources is unknown. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! |
| Concentration | 1.0 mg/mL - lot specific |
| Conjugation | HRP |
| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Database Link | |
| Background | Invertase (saccharase, glucosucrase, beta-h-fructosidase, beta-fructosidase, invertin, sucrase, maxinvert L 1000, fructosylinvertase, alkaline invertase, acid invertase, systematic name: beta-fructofuranosidase) is an enzyme that catalyzes the hydrolysis of sucrose. The resulting mixture of fructose and glucose is called inverted sugar syrup. Related to invertases are sucrases. Invertases and sucrases hydrolyze sucrose to give the same mixture of glucose and fructose. Invertases cleave the O-C (fructose) bond, whereas the sucrases cleave the O-C (glucose) bond. |
| Synonyms | goat anti-Invertase Antibody HRP Conjugated, Peroxidase Conjugated goat anti-Invertase Antibody, Beta fructofuranosidase 2 antibody, Inv1 antibody, Saccharase antibody, Sucrase antibody |
| Note | Anti-Invertase has been tested by ELISA and western blot. This product is assayed against 1.0 ug of Invertase Candida in a standard capture ELISA using ABTS (2,2’-azino-bis-3-ethylbenthiazoline-6-sulfonic acid) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for this product. |
| Reference Data | |
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