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TLN1 Mouse Antibody [Clone ID: 8D4]

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SKU
TA389223
Anti-Talin (Rod domain) Antibody
Size:
100 ul
Conjugation:
Unconjugated
  $425.00
5 Days*
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Specifications
Specifications
Product Data
Clone Name 8D4
Application ICC, IP, WB
Recommended Dilution WB: 1:1000
ICC: 1:100
Reactivity Chicken, Human, Mouse, Rat, Xenopus
Antibody Host Mouse
Isotype IgG1
Immunogen Clone 8D4 was generated from purified chicken gizzard talin. The antibody recognizes an epitope within the rod domain of talin from many species, including human, rat, mouse, chicken, and frog.
Specificity The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells.
Buffer PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Concentration lot specific
Purification Protein A Purified
Conjugation Unconjugated
Storage Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Stability After date of receipt, stable for at least 1 year at -20°C.
Shipping Blue Ice
Predicted Protein Size 240
Database Link
Background Talin is an important cytoskeletal component of integrin adhesion sites. Calpins cleave talin precursor (240 kDa) into an amino-terminal globular head domain of 47 kDa and a carboxyl-terminal 190 kDa rod domain. The talin head domain contains a FERM domain that binds integrins, PIP kinase (Type I), and FAK. The rod domain has several vinculin-binding sites, a second integrin-binding site, and two actin-binding sites. These talin protein-protein interactions are critical for integrin activation, focal adhesion formation, and cell migration. Talin regulation may occur through phosphorylation and regulated degradation. The talin head domain binds Smurf1, an E3 ubiquitin ligase, and this interaction leads to talin head ubiquitylation and degradation. Cdk5 can phosphorylate Ser-425 in the head domain, and this inhibits both binding to Smurf1 and subsequent degradation. The S425A talin mutant resists Cdk5 phosphorylation, increases susceptibility to Smurf1-mediated ubiquitylation, and inhibits cell migration. Thus, talin head phosphorylation may be important for regulating adhesion stability and cell migration
Note Protein G purified tissue culture supernatant.
Reference Data
Protein Categories Intracellular Proteins
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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