Phospho-Phospho (Tyr) (pTyr) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ICC, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:1000 ICC: 1:100 |
| Reactivity | Chicken, Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Anti-Phosphotyrosine was generated from a panel of phosphotyrosine-containing peptide immunogens designed from human protein sequences. All peptide sequences used are highly conserved phosphotyrosine motifs. |
| Specificity | This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells. |
| Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Concentration | lot specific |
| Purification | Antigen Affinity Purified |
| Conjugation | Unconjugated |
| Storage | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Stability | After date of receipt, stable for at least 1 year at -20°C. |
| Shipping | Blue Ice |
| Background | Phosphorylation of specific tyrosine residues is an important post-translational modification for regulating the activity of most proteins. Stimulation of a variety of cell signaling pathways activates the receptor and non-receptor tyrosine kinases that mediate these protein modifications. Antibodies that can detect phosphotyrosine residues are excellent tools for characterizing changes in the post-translational state of a broad range of phosphotyrosine-containing proteins. Immunoprecipitation of proteins of interest, followed by detection of phosphotyrosine using anti-phosphotyrosine antibody is commonly used to correlate changes in tyrosine phosphorylation state with alterations in protein activity. |
| Note | Antigen affinity purified rabbit serum. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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