NOS1 Mouse Antibody [Clone ID: M401]
Specifications
| Product Data | |
| Clone Name | M401 |
|---|---|
| Application | ICC, IHC, WB |
| Recommended Dilution | WB: 1:1000 ICC: 1:100 |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Immunogen | Clone (M401) was generated from a recombinant protein that included amino acid residues within the C-terminal region of human nNOS. This human nNOS sequence has high homology with similar regions in rat and mouse nNOS. |
| Specificity | The antibody detects a 155 kDa* protein on SDS-PAGE immunoblots of rat and mouse brain tissue lysates, as well in the rat pituitary cell line GC. |
| Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Concentration | lot specific |
| Purification | Protein A Purified |
| Conjugation | Unconjugated |
| Storage | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Stability | After date of receipt, stable for at least 1 year at -20°C. |
| Shipping | Blue Ice |
| Predicted Protein Size | 155 |
| Database Link | |
| Background | Nitric oxide (NO) has a broad range of biological activities and is implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOS), the enzymes responsible for synthesis of NO, are homodimers whose monomers are themselves two fused enzymes: a cytochrome reductase and a cytochrome that requires three cosubstrates (L-arginine, NADPH, and oxygen) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin, and heme). Several distinct NOS isoforms are produced from three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS: nNOS (also designated bNOS, NOS-I), whose activity was first identified in neurons and eNOS (also designated ecNOS, NOS-III) first identified in endothelial cells. The inducible form of NOS, iNOS (also designated NOS-II), is Ca2+ independent and is expressed in a broad range of cell types. This form of NOS is induced after stimulation with cytokines and exposure to microbial products. |
| Note | Protein G purified tissue culture supernatant. |
| Reference Data | |
| Protein Categories | Growth Factors, Intracellular Proteins |
Reviews
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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