ATP1B3 Mouse Antibody [Clone ID: M025]
Specifications
| Product Data | |
| Clone Name | M025 |
|---|---|
| Application | ICC, IP, WB |
| Recommended Dilution | WB: 1:1000 ICC: 1:100 |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Immunogen | Clone (M025) was generated from a proprietary antigen related to the native human Na+/K+ ATPase β3 subunit expressed in MeWo melanoma cell line. |
| Specificity | Clone M025 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE "Native" immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase β3 subunit. The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the β3 subunit on live cells. |
| Buffer | PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol |
| Concentration | lot specific |
| Purification | Protein G Purified |
| Conjugation | Unconjugated |
| Storage | Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. |
| Stability | After date of receipt, stable for at least 1 year at -20°C. |
| Shipping | Blue Ice |
| Predicted Protein Size | 40 |
| Database Link | |
| Background | The Na+/K+ ATPase is an integral membrane heterodimer belonging to the P-type ATPase family. This ion channel uses the energy derived from ATP hydrolysis to maintain membrane potential by driving Na+ export and K+ import across the plasma membrane. It is composed of a large catalytic α subunit and a membrane-spanning auxiliary β subunit. In humans, the Na+/K+ ATPase is a binary complex of an α subunit that has four isoforms (α1-α4) and a β-subunit that has three isoforms (β1, β2, β3). Na+/K+ ATPase subunit expression has been shown to be upregulated in cancers, and inhibition of Na+/K+ ATPase activity has anti-cancer effects. The β3 subunit of Na+/K+ ATPase has increased expression in human gastric cancer tissues and cell lines, and its increased expression level predicts poor patient outcome. β3 subunit knockdown significantly inhibited cell proliferation, colony-formation ability, migration, and invasion in human gastric carcinoma cell lines. |
| Note | Protein G purified tissue culture supernatant. |
| Reference Data | |
| Protein Categories | CD Markers, Intracellular Proteins, Membrane Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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