Mouse Monoclonal Antibody [Clone ID: 6F7]

SKU
TA389003
Anti-CRISPR Cas9 Antibody
  $610.00
5 Days*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name 6F7
Application ICC, IHC, WB
Recommended Dilution WB: 1:1000
ICC: 1:500-1:5000
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Recombinant protein from C-terminal region of Cas9 from Staphylococcus aureus.
Specificity Specific for the Staphylococcus aureus Cas9 protein.
Buffer PBS + 50% glycerol and 5 mm NaN3
Concentration lot specific
Purification Protein G Purified
Conjugation Unconjugated
Storage Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Stability After date of receipt, stable for at least 1 year at -20°C.
Shipping Blue Ice
Predicted Protein Size 53
Background CRISPR/Cas9 is a genome editing tool that makes possible the permanent alteration of genes by directing specifically designed RNA sequences complexed with the nuclease Cas9 to cleave DNA at any desired location allowing the deletion or addition of genes. Antibodies against Cas9 are very useful in the direct detection of the CRISPR/Cas9 complex. This antibody detects Cas9 from Staphylococcus aureus transfected into cells and shows no reactivity toward the Streptococcus pyogenes Cas9 protein (Ran et al., 2015).
Note Protein G purified culture supernatant.
Reference Data
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