CCL15 Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | Sandwich ELISA: To detect hMIP-5 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human MIP-5 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hMIP-5. Western Blot To detect hMIP-5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Reactivity | Human |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hMIP-5. Anti-Human MIP-5 specific antibody was purified by affinity chromatography and then biotinylated. |
| Concentration | lot specific |
| Purification | MIP-5 specific antibody was purified by affinity chromatography and then biotinylated |
| Conjugation | Biotin |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | MIP-5 is a CC chemokine that is expressed in the heart, skeletal muscle and adrenal gland. MIP-5 primarily signals through the CCR1 receptor, but has also been found to bind to CCR3. MIP-5 is chemotactic towards T cells and monocytes. Recombinant Human MIP-5 is a 10.1 kDa protein containing 92 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
| Reference Data | |
| Protein Categories | Chemokines, Cytokines, Intracellular Proteins, Secreated Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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