Il3 Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | Sandwich ELISA: To detect mIL-3 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine IL-3 as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mIL-3. Western Blot To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Reactivity | Mouse |
| Antibody Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits immunized with highly pure Recombinant Murine IL-3. Anti-Murine IL-3-specific antibody was purified by affinity chromatography and then biotinylated. |
| Concentration | lot specific |
| Purification | IL-3-specific antibody was purified by affinity chromatography and then biotinylated |
| Conjugation | Biotin |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Murine IL-3 is a 15.1 kDa globular protein containing 135 amino acid residues. |
| Reference Data | |
| Protein Categories | Cytokines, Growth Factors, Secreated Proteins |
Reviews
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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