CCL11 Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, WB |
|---|---|
| Recommended Dilution | Sandwich ELISA: To detect hEotaxin by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human Eotaxin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEotaxin. Western Blot To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
| Reactivity | Human |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hEotaxin. Anti-Human Eotaxin specific antibody was purified by affinity chromatography and then biotinylated. |
| Concentration | lot specific |
| Purification | Eotaxin specific antibody was purified by affinity chromatography and then biotinylated |
| Conjugation | Biotin |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Database Link | |
| Background | Eotaxin is a CC chemokine that signals through the CCR3 receptor. It is produced by IFN-gamma-stimulated endothelial cells and TNF-activated monocytes. Eotaxin selectively chemoattracts eosinophils and, along with Eotaxin-2 and Eotaxin-3, plays a key role in the regulation of eosinophil recruitment in the asthmatic lung and in allergic reactions. Recombinant Human Eotaxin is an 8.3 kDa protein containing 74 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
| Reference Data | |
| Protein Categories | Allergies and autoimmune diseases, Chemokines, Cytokines, Growth Factors, Secreated Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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