Ifng Mouse Monoclonal Antibody [Clone ID: DB-12]
Specifications
| Product Data | |
| Clone Name | DB-12 |
|---|---|
| Recommended Dilution | ELISA, ELISPOT, Intracellular staining, Immunohistochemistry, Western Blot |
| Reactivity | Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | IFN-gamma |
| Buffer | Prior to lyophilization: 0.5 ml PBS + 125 mM trehalose. |
| Concentration | lot specific |
| Purification | Ion exchange chromatography. |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Ambient |
| Predicted Protein Size | 17.9 kDa |
| Gene Name | interferon gamma |
| Database Link | |
| Background | The mouse anti-rat IFN-? monoclonal antibody (clone DB-12) was first described by Van der Meide P.H. et al. in 1989. The rat IFN-? antibody is of the IgG1 subtype and possesses very low neutralizing activity. In Western blot analysis the DB-12 antibody reacts with the 14 kDa and 18 kDa polypeptides present in rat IFN-? with moderate activity and it has shown not to bind with either mouse IFN-? nor human IFN-?. Further, the DB-12 antibody is highly effective as coating antibody in both sandwich ELISA and ELISPOT assays using biotinylated DB-1 antibody for detection. Also the reverse combination in which the DB-1 antibody functions as coating antibody and biotinylated DB-12 antibody as detector can be used in an ELISA system (Haagmans B.L. et al. 1994). The DB-12 antibody is also applicable in immunohistochemistry on frozen tissues and intracellular staining (Nennesmo, I. et al. 1989 and Bernard, I et al. 1998). |
| Synonyms | IFG; IFI; IFN-gamma |
| Reference Data | |
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