RNF70 (PJA1) Rabbit Polyclonal Antibody

CAT#: TA349130

Rabbit Polyclonal Anti-PJA1 Antibody

Product Images

Western blot analysis of PJA1 in human brain tissue lysate with PJA1 antibody at 1 ug/mL.

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  Immunohistochemistry of PJA1 in mouse brain tissue with PJA1 antibody at 5 ug/mL.

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  Immunofluorescence of PJA1 in mouse brain tissue with PJA1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PJA1 antibody was raised against a 19 amino acid peptide near the amino terminus of human PJA1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: PJA1 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 71 kDa; Observed: 71 kDa
• Gene Name: praja ring finger ubiquitin ligase 1
• Database Link:
  NP_660095
  Entrez Gene 64219 Human
  UniProt ID Q8NG27
• Background: Ubiquitinization is an important cellular degradation process requiring sequential reactions that are mediated by three enzymes: E1, E2 and E3. PJA1, also known as Praja1 and RING finger protein 70, is a 643 amino acid E2-dependent E3-ubiquitin ligase that is abundantly expressed in the brain (1,2). Through interaction and activation with the E2-ubiquitin ligase UBC4, PJA1 mediates substrate-specific ubiquitination via its RING finger domain and facilitates ubiquitination (3). Overexpression of PJA1 in gastrointestinal cancers suggests that it may be responsible for the degradation of some anti-oncogenic proteins (4,5).
• Synonyms: BIgR; IGSF4B; Necl-1; NECL1; synCAM3; TSLL1