BAF53b (ACTL6B) Rabbit Polyclonal Antibody

CAT#: TA347076

Rabbit Polyclonal ACTL6B Antibody

Product Images

WB using the antibody against ACTL6B diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 47 kDa) is indicated on the right; the marker (in kDa) is shown on the left.

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  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against ACTL6B. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:76,000.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:500 ?? 1:1,000) ; Western blotting (1:1,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-ACTL6B antibody: human ACTL6B (actin-like 6B), using a KLH-conjugated synthetic peptide containing a sequence from the central part of the protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: actin like 6B
• Database Link:
  NP_057272
  Entrez Gene 51412 Human
  UniProt ID O94805
• Background: ACTL6B (UniProt/Swiss-Prot entry O94805) belongs to the family of actin-related proteins (ARPs) which are involved in diverse cellular processes such as vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6B is a subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is thought to antagonize chromatin-mediated transcriptional repression thereby facilitating transcriptional activation of specific genes. Further, ACTL6B may be involved in gene regulation by structural modulation of their chromatin, specifically in the brain.
• Synonyms: ACTL6; BAF53B

Reference Data

• Protein Families: Druggable Genome