LIF Rat Monoclonal Antibody [Clone ID: 39N7D10]
Specifications
| Product Data | |
| Clone Name | 39N7D10 |
|---|---|
| Application | ICC/IF, IHC, WB |
| Recommended Dilution | Immunohistochemistry, Immunohistochemistry-Paraffin: 5 ug/mL, Western Blot: 3-5ug/ml~, Immunocytochemistry/ Immunofluorescence |
| Reactivity | Human, Mouse |
| Antibody Host | Rat |
| Isotype | IgG2b, kappa |
| Clonality | Monoclonal |
| Immunogen | A recombinant murine Lif protein containing amino acids 24-203 was used as the immunogen for this antibody. |
| Buffer | PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Concentration | lot specific |
| Purification | Protein G purified |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | leukemia inhibitory factor |
| Database Link | |
| Background | Lif has long been recognized as a cytokine which promotes murine germ cell growth in the absence of feeder cell layers in mouse cells (1-3)Characterized initially as an inhibitor of differentiation in M1 murine monocytic leukemia lines (hence the name Leukemia Inhibitory Factor),Lif is now recognized as a key factor for maintaining stem cells in the undifferentiated state- at least in mouse embryonic stem cells (4)Acting through the Lif receptor and the induced activation of STAT3, murine ESCs can be maintained in vitro in undifferentiated stateHowever, human ESCs, although functionally stimulated with Lif, are not similarly maintained as undifferentiatedThis illustrates a different functional program for Lif in mice and humans in maintenance of stem cellsAn IL-6 family member, Lif is an important pleiotropic cytokine with activity on cells of hematopoietic and non-hematopoietic origin. |
| Synonyms | CDF; DIA; HILDA; MLPLI |
| Note | The Lif protein can be highly glycosylated and has been observed between 22 kD and 34 kD in western blotting. In IHC-P application, 1M EDTA buffer pH 9.0 was used for antigen retrieval (citrate buffer pH 6.0 did not work for this target). |
| Reference Data | |
| Protein Categories | Cytokines, Growth Factors, Intracellular Proteins, Secreated Proteins |
| Protein Families | Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
| Protein Pathways | Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway |
Reviews
Documents
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| FAQs |
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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