Hsp60 (HSPD1) Rabbit Polyclonal Antibody

SKU TA336826

Rabbit Polyclonal HSP60 Antibody

Product Data

• Application: FC, ICC/IF, IHC, Immunoblotting, Simple Western, WB
• Recommended Dilution: Immunohistochemistry: 1:100, Western Blot: 0.5 ug/ml, Immunohistochemistry-Paraffin: 1:100, Simple Western: 1:5000, Flow Cytometry, Immunoblotting, Knockdown Validated, Immunocytochemistry/ Immunofluorescence: 1:50-1:1000
• Reactivity: Hamster, Human, Mouse, Primate, Rat
• Antibody Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an internal region of the human Hsp60 protein (within residues 70-150). [Swiss-Prot P10809]
• Buffer: PBS with 30% Glycerol, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: heat shock protein family D (Hsp60) member 1
• Database Link:
  NP_002147
  P10809
• Background: Heat shock protein 60 activates T cells and ultimately autoimmune actions and diseases. In gastric cancer, HSP60 is upregulated in malignant ascites that have been treated, and induces a tumor-specific cytotoxic T lymphocyte response. Enhancing Hsp60 expression is beneficial for protecting bone tissue against osteoblast apoptosis. Hsp60 is highly influenced by dietary intake of antioxidants, and is a prime target for many natural therapies against gut inflammation, preeclampsia, and some low level bacterial infections. Ischemia and reperfusion, as well as hypoxic environments can cause temporary elevations in Hsp60 expression levels.
• Synonyms: CPN60; GROEL; HLD4; HSP-60; HSP60; HSP65; HuCHA60; SPG13
• Note: This Hsp60 antibody is useful for Immunocytochemistry/Immunofluorescence, Western Blot, and IHC-paraffin embedded sections. In Western Blot, a band is seen ~61 kDa representing Hsp60. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: RNA degradation, Type I diabetes mellitus