BIRC5 Mouse Monoclonal Antibody [Clone ID: 8E2]
Specifications
| Product Data | |
| Clone Name | 8E2 |
|---|---|
| Application | Block/Neutralize, ELISA, FC, ICC/IF, IHC |
| Recommended Dilution | Block/Neutralize, ELISA, Flow Cytometry: 1 ug/mL, Flow (Intracellular), Immunohistochemistry: 1:100, Immunocytochemistry/ Immunofluorescence: 1:50-1:100, Immunohistochemistry-Paraffin: 1:100, In vitro assay |
| Reactivity | Human, Rat |
| Antibody Host | Mouse |
| Isotype | IgG1, kappa |
| Clonality | Monoclonal |
| Immunogen | Full length recombinant human Survivin UniProt# O15392 |
| Buffer | PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Concentration | lot specific |
| Purification | Protein G purified |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | baculoviral IAP repeat containing 5 |
| Database Link | |
| Background | Survivin (BIRC5 or IAP4) is the smallest member of inhibitors of apoptosis proteins (X-linked IAP/XIAP, c-IAP1, c-IAP2, IAP-like protein-2/ ILP2, melanoma IAP/ ML-IAP, Livin, NAIP, and survivin) and besides its anti-apoptotic functions; survivin also regulates processes such as cell cycle, migration, metastasis etc. Highly critical in fetal development, survivin is generally no longer present in adult tissues; however, its re-expression occurs in cancers wherein it drives proliferation, metastasis, poor prognosis, and decreased patient survival. Survivin contains only one copy of a modified baculovirus IAP repeat (BIR, a domain required for caspases inactivation) which is used for homodimerization and for its interaction with other chromosome passenger proteins. Phosphorylation/mutation of Thr-48 in the BIR domain affects survivin's dual ability to inhibit apoptosis and regulate cell division. Under apoptotic stimuli involving mitochondria, survivin forms a complex with XIAP, thus increasing the stability of XIAP and its inhibitory activity against caspases. The survivin binding to mitochondrial Smac/DIABLO leads to a delayed Smac/DIABLO release in the cytoplasm resulting in prolonged cell survival. Survivin-HBXIP complex can bind to pro-caspase-9 for exerting prevention of mitochondrial apoptosis activation. For facilitating correct cell division, survivin recognize/complex with phospho-histone-H3 and associates with the chromosome passenger proteins (Aurora B, INCENP, and borealin) and this new complex is recruited to the mitotic centromers where it assists/ensure proper chromosomal segregation. Moreover, at the centrosomes of dividing cells, survivin binds to Cdk1 which is critical for cells to enter mitosis. |
| Synonyms | API4; EPR-1 |
| Note | This Survivin Antibody (8E2) is useful for Immunocytochemistry, Immunofluorescence and Immunohistochemistry on paraffin embedded sections. For IHC, heat induced antigen retreval using citrate buffer is required. In vitro assay, Blocking/Neutralizing, ELISA, Western Blot, and Flow Cytometry were reported in scientific literature. |
| Reference Data | |
| Protein Categories | Cytokines, Intracellular Proteins |
| Protein Families | Druggable Genome, Stem cell - Pluripotency |
| Protein Pathways | Colorectal cancer, Pathways in cancer |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
