Caspase 8 (CASP8) Mouse Monoclonal Antibody [Clone ID: FLICE 4-1-20]

CAT#: TA336653

Mouse Monoclonal Caspase-8 Antibody (FLICE 4-1-20)

Product Images

Western Blot: Caspase 8 Antibody (FLICE 4-1-20) TA336653 - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using TA336653 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.

Specifications

Product Data

• Clone Name: FLICE 4-1-20
• Applications: WB
• Recommended Dilution: Western Blot: 1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Full-length recombinant human Caspase 8 [UniProt# Q14790]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: caspase 8
• Database Link:
  NP_203519
  Entrez Gene 841 Human
  UniProt ID Q14790
• Background: Caspases are evolutionary conserved family of aspartate-specific cystein proteases which are essential part of apoptotic machinery and typically signal in a two-step cascade: 'initiator caspases' with large prodomains (such as caspase-1, -8 and -9) are first recruited into large protein complexes in which they undergo proximity-induced autoactivation and "executioner caspases" such as caspase-3 and -6, caspase-7 which are activated by initiator caspases via freeing of executioner caspases of their short inhibitory prodomain, allowing them to cleave a large set of cellular substrates. Localized in cytoplasm, caspase 7 is a heterotetramer that is consists of two anti-parallel arranged heterodimers, each one formed by a 20 kD (p20) and a 11 kD (p11) subunit, and interacts with BIRC6/bruce. Caspase 7 cleaves/activates SREBPs and poly(ADP-ribose) polymerase (PARP). Its overexpression promotes apoptosis, and it also implicates in inflammation signaling. Therapeutic intervention with caspase-7 has been shown to prove beneficial in conditions where excessive cell death and/or inflammation contribute to diseases e.g. Alzheimer's disease, Huntington's disease, sepsis, rheumatoid arthritis, cancer and NIDDM.
• Synonyms: ALPS2B; CAP4; Casp-8; FLICE; MACH; MCH5
• Note: This Caspase 8 (FLICE 4-1-20) antibody is useful for Western Blot where a band is seen at 55 kDa representing the inactive form of Caspase 8 (see protocol for additional information). We also have reports that the antibody detects the active (pro) form of Caspase 8.

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease, Apoptosis, Huntington's disease, NOD-like receptor signaling pathway, p53 signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Viral myocarditis