APIP Mouse Monoclonal Antibody [Clone ID: 19F461]

SKU TA336411

Mouse Monoclonal APIP Antibody (19F461)

Product Data

• Clone Name: 19F461
• Application: IHC, WB
• Recommended Dilution: Immunohistochemistry-Paraffin, Immunohistochemistry: 5 ug/ml, Western Blot: 1-2 ug/ml
• Reactivity: Human
• Antibody Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: The antibody was developed against a full-length His-tagged recombinant APIP.
• Buffer: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Gene Name: APAF1 interacting protein
• Database Link:
  NP_057041
  Q96GX9
• Background: The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis3. Recently, Cho et al have identified a new Apaf-1 Interactiong Protein (APIP) also known as CG129 and MMRP19, as a negative regulator of ischemic injury. APIP competes with Caspase-9 binding site of Apaf1. APIP is predicted to code for a 204 amino acid. An isoform of APIP, APIP2 encodes a 242 amino acid protein, which is an alternative splicing variant differing in its N-terminus from APIP. APIP transcript is ubiquitously expressed in most adult tissue with high expression in skeletal muscle, heart, and kidney.
• Synonyms: APIP2; CGI-29; CGI29; hAPIP; MMRP19

Reference Data

• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways