Heme Oxygenase 1 (HMOX1) Mouse Monoclonal Antibody [Clone ID: 1F12-A6]

CAT#: TA326354

Mouse monoclonal HO-1 Antibody

Product Images

Western blot analysis of HO-1 using a 1:1000 dilution of the antibody

Specifications

Product Data

• Clone Name: 1F12-A6
• Applications: WB
• Recommended Dilution: WB: 1:500-1000
• Reactivities: Human, Mouse, Bovine
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: Human HO-1 synthetic peptide, amino acids 1-30
• Formulation: PBS pH7.4, 50% glycerol
• Concentration: lot specific
• Purification: Protein G Purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: heme oxygenase 1
• Database Link:
  NP_002124
  Entrez Gene 15368 MouseEntrez Gene 3162 Human
  UniProt ID P09601
• Background: Heme-oxygenase is a ubiquitous enzyme that catalyzes the initial and rate-limiting steps in heme catabolism yielding equimolar amounts of biliverdin, iron and carbon monoxide. Biliverdin is subsequently converted to bilirubin and the free iron is sequestered to ferritin . These products have important physiological effects as carbon monoxide is a potent vasodilator; biliverdin and bilirubin are potent antioxidants; and the free iron increases oxidative stress and regulates the expression of many mRNAs .There are three isoforms of heme-oxygenase, HO-1, HO-2 and HO-3; however HO-1 and HO-2 are the major isoforms as they both have been identified in mammals . HO-1, also known as heat shock protein 32, is an inducible isoform activated by most oxidative stress inducers, cytokines, inflammatory agents and heat shock. HO-2 is a constitutive isoform which is expressed under homeostatic conditions. HO-1 is also considered to be a cytoprotective factor in that free heme is highly reactive and cytotoxic, and secondly, carbon monoxide is a mediator inhibiting the inflammatory process and bilirubin is a scavenger for reactive oxygen, both of which are the end products of heme catalyzation . It has also been shown that HO-1 deficiency may cause reduced stress defense, a pro-inflammatory tendency , susceptibility to atherosclerotic lesion formation , endothelial cell injury, and growth retardation . Up-regulation of HO-1 is therefore said to be one of the major defense mechanisms of oxidative stress .
• Synonyms: bK286B10; HMOX1D; HO-1; HSP32
• Note: Detects a 32kDa protein, corresponding to the molecular mass of HO-1 on SDS Page immunoblots. Does not cross-react with HO-2

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Porphyrin and chlorophyll metabolism