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Phospho Smad2/3 (Thr8) Rabbit Polyclonal Antibody

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SKU
TA325852
Rabbit Polyclonal Smad2/3 (Thr8) Antibody (Phospho-specific)
Size:
100 ul
Conjugation:
Unconjugated
  $335.00
4 Weeks*
Bulk/Customize

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View other "Phospho Smad2/3 (Thr8) or NM_001145102" antibodies

Specifications
Specifications
Product Data
Application WB
Recommended Dilution WB: 1:500-1:2000
Reactivity Human, Mouse, Rat
Modification Phospho-specific
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen The antiserum was produced against A synthesized peptide derived from human Smad2/3 around the phosphorylation site of Threonine 8
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration lot specific
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Predicted Protein Size 48/60 kDa
Gene Name SMAD family member 3
Database Link
Background Smad2 ubiquitously expressed transcription factor phosphorylated and activated by TGF-beta receptor-type kinases. Participates in a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis.
Synonyms HSPC193; HsT17436; JV15-2; LDS1C; LDS3; MADH3
Reference Data
Protein Categories Growth Factors, Intracellular Proteins, Transciption Factors
Protein Families Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Transcription Factors
Protein Pathways Adherens junction, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Pancreatic cancer, Pathways in cancer, TGF-beta signaling pathway, Wnt signaling pathway
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Documents
Resources

file-alt Citations

Long-Term Protective Effects of Succinate Dehydrogenase Inhibition during Reperfusion with Malonate on Post-Infarction Left Ventricular Scar and Remodeling in Mice
Valls-Lacalle, Consegal, Ganse et al
Int J Mol Sci (2024) 25 (8) DOI: 10.3390/ijms25084366
Cited by 8
AI Summary: In this study, the OriGene antibody pSMAD2/3 (Thr8) (#TA325852) was used to assess the activation of fibrotic signaling pathways, contributing to the evaluation of pro-inflammatory and fibrotic responses following myocardial infarction and malonate treatment in mice.
Snippet: of markers involved in the pro-inflammatory and fibrotic responses, including NF-κB p65 (#ab16502, abcam, 1:1000), Smad2/3 (#sc-398844, Santa Cruz Biotechnology, 1:500), pSMAD2/3 (Thr8) (#TA325852, OriGene, 1:1000), transforming growth factor β1 (TGFβ1) (#ab92486, abcam, 1:1000), connective tissue growth factor (CTGF) (#ab6992, abcam, 1:1000), fibroblast activation factor α (FAPα) (#PIPA5120
FigureFigureFigure
Connexin 43 Deficiency Is Associated with Reduced Myocardial Scar Size and Attenuated TGFβ1 Signaling after Transient Coronary Occlusion in Conditional Knock-Out Mice
Valls-Lacalle, Consegal, Ruiz-Meana et al
Biomolecules (2020) 10 (4) DOI: 10.3390/biom10040651
Cited by 10
AI Summary: OriGene contributed to this study by providing the anti-phospho-Smad2/3 (Thr8) antibody (#TA325852), which was used to assess TGFβ1 signaling in myocardial tissue.
Snippet: antibodies were used: rabbit anti-NF-κB p65 (#ab16502, Abcam, dilution 1:1000), anti-Smad2/3 (total forms: #sc398844 (raised in mice), Santa Cruz Biotechnology, Dallas, TX, USA, 1:500;phosphorylated forms (Thr8): #TA325852 (raised in rabbit), Origene, Rockville Cached;- antibodies were used: rabbit anti-NF-κB p65 (#ab16502, Abcam, dilution 1:1000), anti-Smad2/3 (total forms: #sc398844 (raised in mice), Santa Cruz Biotechnology, Dallas, TX, USA, 1:500
Histidine and arginine modulate intestinal cell restitution via transforming growth factor-β1
Matsui, Ichikawa, Fujita et al
Eur J Pharmacol (2019) 850, 35-42 DOI: 10.1016/j.ejphar.2019.02.006
Cited by 20
AI Summary: In this study, OriGene’s Tgf-β1 (Cat# SR307914) and Gapdh (Cat# SR412874) qPCR primers were used to quantify mRNA levels, helping demonstrate that histidine and arginine deficiency reduces Tgf-β1 expression and impairs intestinal cell restitution.
RAGE Deletion Confers Renoprotection by Reducing Responsiveness to Transforming Growth Factor-β and Increasing Resistance to Apoptosis
Hagiwara, Sourris, Ziemann et al
Diabetes (2018)
AI Summary: OriGene’s Smad3 antibody (used at 1:5000) was utilized in this study to assess Smad3 protein levels, contributing to the investigation of molecular pathways affected by RAGE deletion in diabetic nephropathy.
Snippet: Primary antibodies were collagen I (1:5000;Abcam), αSMA (1:2000;Dako), phospho-smad3 (1:1000;Invitrogen), smad3 (1:5000;Origene), phospho-ERK (1:500;Cell Signaling), ERK (1:1000;Cell Signaling), S100A4 (1:1000;Cell signaling), P53 (1:500;Abcam)
Delayed, oral pharmacological inhibition of calpains attenuates adverse post-infarction remodelling
Poncelas, Inserte, Aluja et al
Cardiovasc Res (2017) 113 (8), 950-961 DOI: 10.1093/cvr/cvx073
Cited by 38
AI Summary: In this study, OriGene products—specifically anti-Calpain-1 (Cat# TA324872) and anti-Calpain-2 (Cat# TA324873) antibodies—were used to measure calpain protein levels in heart tissue, helping to demonstrate the effectiveness of calpain inhibition in reducing adverse post-infarction cardiac remodeling.
Showing 1-5 of 5 papers.
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