Actin (ACTA1) Rabbit Polyclonal Antibody

CAT#: TA324566

Rabbit polyclonal ACTA1/Alpha-actin Antibody (C-term)

Product Images

ACTA1/α-actin Antibody (C-term) (Cat.# TA324566) western blot analysis in A549, RD cell line and human placenta lysates (35ug/lane).This demonstrates the ACTA1/α-actin antibody detected the ACTA1/α-actin protein (arrow).

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  ACTA1/α-actin Antibody (C-term) (TA324566)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ACTA1/α-actin Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

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  Confocal immunofluorescent analysis of ACTA1/α-actin Antibody (C-term) (Cat#TA324566) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

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  ACTA1/α-actin Antibody (C-term) (Cat. #TA324566) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: WB: 1:1000, IHC: 1:10~50, IF: 1:10~50, FC: 1:10~50
• Reactivities: Human (Predicted: Mouse, Rat, Bovine, Chicken, Pig, Rabbit, Xenopus)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: This ACTA1/Alpha-actin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the C-terminal region of human ACTA1/Alpha-actin.
• Formulation: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: lot specific
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42051 Da
• Gene Name: actin, alpha 1, skeletal muscle
• Database Link:
  NP_001091
  Entrez Gene 11459 MouseEntrez Gene 29437 RatEntrez Gene 58 Human
  UniProt ID P68133
• Synonyms: ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; SHPM

Reference Data

• Protein Families: Stem cell - Pluripotency