Nck (NCK1) Rabbit Polyclonal Antibody

CAT#: TA324087

Anti-NCK1 Rabbit Polyclonal Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane: Hela cells Primary antibody: TA324087 (NCK1 Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA324087 (NCK1 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA324087 (NCK1 Antibody) at dilution 1/20, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA324087 (NCK1 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA324087 (NCK1 Antibody) at dilution 1/20, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hela cells
  IHC: 10-50
  Positive control: Human lung cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein corresponding to a C terminal 300 amino acids of human NCK adaptor protein 1
• Formulation: PBS pH7.3, 0.05% NaN3, 50% glycerol
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: NCK adaptor protein 1
• Database Link:
  NP_001177725
  Entrez Gene 17973 MouseEntrez Gene 4690 Human
  UniProt ID P16333
• Background: The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
• Synonyms: NCK; nck-1; NCKalpha

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, ErbB signaling pathway, Pathogenic Escherichia coli infection, T cell receptor signaling pathway