DUOX1 Goat Polyclonal Antibody

CAT#: TA320203

Goat Polyclonal Anti-DUOX1 Antibody

Product Images

Anti-DUOX1 (0.1ug/ml) staining of Human Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.1-0.3ug/ml, ELISA: 1:128,000
• Reactivities: Human
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence CSRSDIETELTPQR (aa972-985), from the internal region of the protein sequence according to NP_059130.2
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 177 kDa
• Gene Name: dual oxidase 1
• Database Link:
  NP_059130
  Entrez Gene 53905 Human
  UniProt ID Q9NRD9
• Background: The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes proteins encoded by this gene and the similar DUOX2 gene. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. This protein generates hydrogen peroxide and thereby plays a role in the activity of thyroid peroxidase, lactoperoxidase, and in lactoperoxidase-mediated antimicrobial defense at mucosal surfaces. Two alternatively spliced transcript variants encoding the same protein have been described for this gene.
• Synonyms: LNOX1; NOXEF1; THOX1
• Note: Approx 170kDa band observed in Human Testis lysates (calculated MW of 177kDa according to NP_059130.2). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide.

Reference Data

• Protein Families: Druggable Genome, Transmembrane