AP1G1 Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | ELISA, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:2,000 ELISA: 1:2,000 - 1:8,000 IP: 1:100 |
| Reactivity | Human |
| Antibody Host | Goat |
| Clonality | Polyclonal |
| Immunogen | This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 625-675 of Human AP1G1. |
| Specificity | This affinity purified antibody is directed against human AP1G1. The product was affinity purified from antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. Cross reactivity with AP1G1 protein from mouse, rat, dog and chicken may occur as sequence homology varies by only one amino acid residue in this sequence as indicated by BLAST analysis. Reactivity with AP1G1 proteins from other sources is not known. |
| Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 0.7 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | adaptor related protein complex 1 gamma 1 subunit |
| Database Link | |
| Background | AP1G1 (also known as gamma 1 subunit of adaptor -related protein complex 1, Gamma-adaptin, Adaptor protein complex AP-1 gamma-1 subunit, Golgi adaptor HA1/AP1 adaptin gamma-1 subunit and Clathrin assembly protein complex 1 gamma-1 large chain) is a subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules. The adaptin family of proteins is composed of four classes of molecules named alpha, beta-, beta prime- and gamma- adaptins. Adaptins, together with medium and small subunits, form a heterotetrameric complex called an adaptor, whose role is to promote the formation of clathrin-coated pits and vesicles. AP1G1 is localized to the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex and is widely expressed. |
| Synonyms | goat anti-AP1G1 Antibody, AP-1 complex subunit gamma-1, Adapter-related protein complex 1 subunit gamma-1, Adaptor protein complex AP-1 subunit gamma-1, Golgi adaptor HA1/AP1 adaptin subunit gamma-1, Gamma1-adaptin, Clathrin assembly protein complex 1 gamma-1 large chain, ADTG, CLAPG1, AP1G |
| Note | This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91 kDa in size corresponding to AP1G1 by western blotting in the appropriate cell lysate or extract. |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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