CUL3 Rabbit Polyclonal Antibody
$870.00
5 Days*
Specifications
| Product Data | |
| Application | ELISA, IHC, IP, WB |
|---|---|
| Recommended Dilution | WB: 1:500 - 1:1,000 IHC: User Optimized ELISA: 1:2,000 - 1:10,000 IP: 1:100 |
| Reactivity | Human |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Cul3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the N-Terminal near amino acids 1-25 of Human Cul3 coupled to KLH. |
| Specificity | Anti-Cul3 antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 3. Cross reactivity is expected against mouse Cul3 based on sequence homology. Cross reactivity with other human cullins is unlikely based on sequence homology. |
| Buffer | Stabilizer: None Preservative: 0.01% (w/v) Sodium Azide |
| Concentration | 85 mg/mL - lot specific |
| Conjugation | Unconjugated |
| Storage | Store anti-Cul3 at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
| Stability | Expiration date is one (1) year from date of receipt. |
| Shipping | Blue Ice |
| Gene Name | cullin 3 |
| Database Link | |
| Background | Cul3 antibody is ideal for Cell Biology, Cancer and Ubiquitin research. Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 3 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul3 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul3 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Unlike Cul1 and Cul2, Cul3 seems not to be a part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2. Cul3 also interacts with RNF7 and is part of a complex with TIP120A/CAND1, Cyclin E and RBX1. |
| Synonyms | rabbit anti-Cul3 Antibody, rabbit anti-Cullin3 antibody, KIAA0617 antibody, CUL, Cullin |
| Note | Anti-Cul3 has been tested by immunohistochemistry. This antibody reacts with human Cul3 by western blot and immunoprecipitation. The antibody immunoprecipitates in vitro translated product and protein from cell lysates (using HeLa or NIH-3T3). An 88.9 kDa band corresponding to human Cul3 is detected. Most cell lines expressing Cul3 can be used as a positive control. Researchers should determine optimal titers for other applications. |
| Reference Data | |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
