OGG1 Rabbit Polyclonal Antibody

CAT#: TA309779

Rabbit Polyclonal Ogg1 Antibody

Product Images

Flow (Intracellular): OGG1 Antibody TA309779 - An intracellular stain was performed on HeLa cells with [TA309779C] (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 650.

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  Flow Cytometry: OGG1 Antibody TA309779 - An intracellular stain was performed on Jurkat Cells with TA309779 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.

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  The protein expression levels of HVL and LVL sorted cells by western blotting.(A) The ATM, phospho-ATM, GPX2, MRE11, OGG1, and XPC levels were measured by western blot analysis. The figure is representative of three independent experiments. (B) The relative fold activity was quantified using AlphaImage software and normalized to the expression of the internal actin control. *P < 0.05, **P < 0.01.

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  Immunocytochemistry/Immunofluorescence: OGG1 Antibody TA309779 - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-hOgg1 TA309779 at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha Tubulin (DM1A) [NB100-690] was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunohistochemistry: OGG1 Antibody TA309779 - Staining of human tonsil, germinal center and mantle zone.

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  Flow Cytometry: OGG1 Antibody TA309779 - An intracellular stain was performed on HeLa cells with OGG1 Antibody TA309779 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

• Western Blot: OGG1 Antibody TA309779 - - Analysis of Ogg1 expression in Jurkat whole cell lysate.

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Specifications

Product Data

• Applications: ELISA, FC, ICC/IF, IHC, Immunoblotting, IP, WB
• Recommended Dilution: ELISA, Immunohistochemistry-Frozen: 1:25-1:100, Immunohistochemistry, Flow (Intracellular), Immunohistochemistry-Paraffin, Immunoprecipitation: 1:10-1:500, Immunoblotting, Flow Cytometry, Western Blot: 1:500-1:1000, Immunocytochemistry/ Immunofluorescence: 1:100 - 1:200
• Reactivities: Human, Primate, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A peptide derived from the human Ogg1 (within amino acids 1-100).
• Formulation: Tris-citrate/phosphate buffer, pH 7 to 8 and 0.09% Sodium Azide
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: 8-oxoguanine DNA glycosylase
• Database Link:
  NP_058213
  Entrez Gene 81528 RatEntrez Gene 4968 Human
  UniProt ID O15527
• Synonyms: HMMH; HOGG1; MUTM; OGH1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair