DNase I (DNASE1) Rabbit Polyclonal Antibody

CAT#: TA308546

Rabbit Polyclonal antibody to DNase I (deoxyribonuclease I)

Product Images

Sample (30 ug of whole cell lysate). A: MOLT4. 10% SDS PAGE. TA308546 diluted at 1:1000

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  Immunofluorescence analysis of methanol-fixed HeLa, using DNase I (TA308546) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Dog)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 282 of DNase I (Uniprot ID#P24855)
• Formulation: 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 31 kDa
• Gene Name: deoxyribonuclease I
• Database Link:
  NP_005214
  Entrez Gene 403413 DogEntrez Gene 1773 Human
  UniProt ID P24855
• Background: This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq]
• Synonyms: DNL1; DRNI
• Note: Seq homology of immunogen across species: Dog (81%)

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane