NXF1 Rabbit Polyclonal Antibody

CAT#: TA308497

Rabbit polyclonal antibody to TAP (nuclear RNA export factor 1)

Product Images

Sample (30 ug of whole cell lysate). A: Hela. B: Molt-4. 7.5% SDS PAGE. TA308497 diluted at 1:1000.

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  Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using TAP (TA308497) antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TAP (TA308497) antibody at 1:200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human (Predicted: Mouse, Rat)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 151 of TAP (Uniprot ID#Q9UBU9)
• Formulation: 1XPBS, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 70 kDa
• Gene Name: nuclear RNA export factor 1
• Database Link:
  NP_001074960
  Entrez Gene 53319 MouseEntrez Gene 59087 RatEntrez Gene 10482 Human
  UniProt ID Q9UBU9
• Background: This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq]
• Synonyms: MEX67; TAP
• Note: Seq homology of immunogen across species: Mouse (87%), Rat (90%)