NDUFS1 Rabbit Polyclonal Antibody

CAT#: TA308300

Rabbit Polyclonal antibody to NDUFS1 (NADH dehydrogenase (ubiquinone) Fe-S protein 1, 75kDa (NADH-coenzyme Q reductase))

Product Images

Sample (50 ug of whole cell lysate). A: Mouse brain. 7.5% SDS PAGE. TA308300 diluted at 1:1000.

• 

  Sample (50 ug of whole cell lysate)Â. Â A: Rat brain. Â 12% SDS PAGEÂ. Â TA308300 diluted at 1:1000. Â Â

• 

  Sample (30 ug of whole cell lysate). A: Molt-4. 12% SDS PAGE. TA308300 diluted at 1:1000.

• 

  Immunohistochemical analysis of paraffin-embedded Colon ca, using NDUFS1 (TA308300) antibody at 1:250 dilution.

• 

  Immunofluorescence analysis of methanol-fixed A431, using NDUFS1 (TA308300) antibody at 1:200 dilution.

Show all 5 images

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:500-1:3000
• Reactivities: Human, Mouse (Predicted: Chimpanzee)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 432 and 664 of NDUFS1 (Uniprot ID#P28331)
• Formulation: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 28 kDa
• Gene Name: NADH:ubiquinone oxidoreductase core subunit S1
• Database Link:
  NP_004997
  Entrez Gene 227197 MouseEntrez Gene 4719 Human
  UniProt ID P28331
• Background: The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. [provided by RefSeq]
• Synonyms: CI-75k; CI-75Kd; PRO1304
• Note: Seq homology of immunogen across species: Chimpanzee (100%)

Reference Data

• Protein Pathways: Alzheimer's disease, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease