Pyrophosphatase 1 (PPA1) Rabbit Polyclonal Antibody

CAT#: TA308035

Rabbit polyclonal antibody to PPA1 (pyrophosphatase (inorganic) 1)

Product Images

PPA1 antibody detects PPA1 protein by Western blot analysis. A. 30 ug Neuro2A whole cell lysate/extract. B. 30 ug GL261 whole cell lysate/extract. C. 30 ug C8D30 whole cell lysate/extract. D. 30 ug NIH-3T3 whole cell lysate/extract. E. 30 ug BCL-1 whole c

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  PPA1 antibody detects PPA1 protein by Western blot analysis. A. 30 ug PC-12 whole cell lysate/extract. B. 30 ug Rat2 whole cell lysate/extract. 12 % SDS-PAGE. PPA1 antibody (TA308035) dilution: 1:1000

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  Sample (30 ug of whole cell lysate). A: H1299. B: Hela. C: Hep G2. D: Molt-4. 12% SDS PAGE. TA308035 diluted at 1:10000

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  Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using PPA1 (TA308035) antibody at 1:500 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using PPA1 (TA308035) antibody at 1:200 dilution.

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Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: ICC/IF:1:100-1:1000; IHC:1:100-1:1000; WB:1:5000-1:20000
• Reactivities: Human (Predicted: Mouse, Rat, Bovine)
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1 and 289 of PPA1 (Uniprot ID#Q15181)
• Formulation: 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
• Concentration: lot specific
• Purification: Purified by antigen-affinity chromatography.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33 kDa
• Gene Name: pyrophosphatase (inorganic) 1
• Database Link:
  NP_066952
  Entrez Gene 67895 MouseEntrez Gene 294504 RatEntrez Gene 5464 Human
  UniProt ID Q15181
• Background: The protein encoded by this gene is a member of the inorganic pyrophosphatase (PPase) family. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Studies of a similar protein in bovine suggested a cytoplasmic localization of this enzyme. [provided by RefSeq]
• Synonyms: HEL-S-66p; IOPPP; PP; PP1; SID6-8061
• Note: Seq homology of immunogen across species: Mouse (94%), Rat (93%), Bovine (95%)

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Oxidative phosphorylation