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PAK2 Rabbit Polyclonal Antibody

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SKU
TA306346
Rabbit Polyclonal PAK2 Antibody
Size:
100 ug
Conjugation:
Unconjugated
  $640.00
In Stock*
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Specifications
Specifications
Product Data
Application IF, IHC, WB
Recommended Dilution WB: 0.5 - 2 ug/mL, ICC: 10 ug/mL, IF: 20 ug/mL
Reactivity Human, Mouse, Rat
Antibody Host Rabbit
Isotype IgG
Clonality Polyclonal
Immunogen PAK2 antibody was raised against a 14 amino acid peptide from near the amino terminus of human PAK2.
Buffer PBS containing 0.02% sodium azide.
Concentration 1ug/ul
Purification Affinity chromatography purified via peptide column
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Shipping Blue Ice
Gene Name p21 (RAC1) activated kinase 2
Database Link
Background The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.
Synonyms PAK65; PAKgamma
Reference Data
Protein Categories Enzyme: Kinases, Growth Factors, Intracellular Proteins
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Axon guidance, ErbB signaling pathway, Focal adhesion, MAPK signaling pathway, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway
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Documents
Resources

file-alt Citations

P21-activated kinase 2 is essential in maintenance of peripheral Foxp3+ regulatory T cells
Choi, Pease, Chen et al
Immunology (2018) 154 (2), 309-321 DOI: 10.1111/imm.12886
AI Summary: OriGene’s anti-Pak2 antibody was used in this study to detect and analyze Pak2 protein levels by immunoblotting, helping to demonstrate Pak2’s essential role in maintaining peripheral Foxp3+ regulatory T cells.
Snippet: …The membranes were incubated with primary antibodies against Pak2 (Origene, Rockville, MD), phospho‐p70S6K (Thr389, Cell Signaling, Danvers, MA), phospho‐S6 (Ser235/236, Cell Signaling), phospho‐extracellular signal‐regulated kinase (ERK) (Thr202/Tyr204, Cell Signaling), phospho‐phospholipase C‐γ (PLC‐γ) (Tyr783, Invitrogen), PLCγ (Cell Signaling), phospho‐guanine nucleotide exchange factor‐H1 (GEF‐H1) (Ser885, Abcam, Cambridge, UK), phospho‐LIM domain kinase 1/2 (LIMK1/2) (Thr508/Thr505, Cell Signaling), phospho‐myosin light chain 2 (MLC2) (Thr18/Ser19, Cell Signaling), phospho‐cofilin (Ser3, Abcam), cofilin (Cell Signaling) and GAPDH (Millipore). …
FigureFigureFigure
WB
Pak2 is required for actin cytoskeleton remodeling, TCR signaling, and normal thymocyte development and maturation
Phee, Au-Yeung, Pryshchep et al
Elife (2014) 3, e02270
AI Summary: OriGene’s Pak2 (TA306346) antibody was used in this study to investigate the role of Pak2 in actin cytoskeleton remodeling, TCR signaling, and thymocyte development and maturation.
Snippet: …Phospho-PLCγ1 (Y783)(44-696G) antibody was purchased from Life Technologies (Grand Island, NY). Pak2 (TA306346) antibody is from Origene (Rockville, MD). …
FigureFigureFigure
WB
Showing 1-2 of 2 papers.
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