PIST (GOPC) Rabbit Polyclonal Antibody

CAT#: TA306263

Rabbit Polyclonal PIST Antibody

Product Images

Western blot analysis of PIST in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/ml.

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  Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 ug/ml.

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  Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 10 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 4 ug/mL, ICC: 1 ug/mL, IF: 10 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: PIST antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human PIST.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1ug/ul
• Purification: Affinity chromatography purified via peptide column
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: golgi-associated PDZ and coiled-coil motif containing
• Database Link:
  AAG00572
  Entrez Gene 57120 Human
  UniProt ID Q9HD26
• Background: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (4-6). Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1 (6).
• Synonyms: CAL; dJ94G16.2; FIG; GOPC1; PIST