Nephrin (NPHS1) Rabbit Polyclonal Antibody

SKU TA306037

Rabbit Polyclonal Nephrin Antibody

Product Data

• Application: IF, IHC, WB
• Recommended Dilution: WB: 1-4 µg/mL; IHC-P: 1-2 µg/mL. ; ; Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse, and rat samples. All other applications and species not yet tested.
• Reactivity: Human, Mouse, Rat
• Antibody Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Anti-Nephrin antibody (2265) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. ; The immunogen is located within the last 50 amino acids of Nephrin.
• Buffer: Nephrin Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Purification: Nephrin Antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Nephrin antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Stability: Stable for 12 months from date of receipt.
• Shipping: Blue Ice
• Predicted Protein Size: Predicted: 135 kD + 10 N-linked glycosylation sites ; ; Observed: 200 kD
• Gene Name: NPHS1 nephrin
• Database Link:
  NP_004637
  O60500
• Background: Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm.
• Synonyms: CNF; nephrin; NPHN
• Note: Optimal dilutions for each application to be determined by the researcher.

Reference Data

• Protein Families: Druggable Genome, Transmembrane