Nephrin (NPHS1) Rabbit Polyclonal Antibody
Specifications
| Product Data | |
| Application | IHC, WB |
|---|---|
| Recommended Dilution | WB: 1-4 µg/mL IHC-P: 1-2 µg/mL. Antibody validated: Western Blot in human, mouse and rat samples Immunohistochemistry in mouse, and rat samples. All other applications and species not yet tested. |
| Reactivity | Human, Mouse, Rat |
| Antibody Host | Rabbit |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Anti-Nephrin antibody (2265) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin. |
| Buffer | Nephrin Antibody is supplied in PBS containing 0.02% sodium azide. |
| Concentration | 1 mg/mL |
| Purification | Nephrin Antibody is affinity chromatography purified via peptide column. |
| Conjugation | Unconjugated |
| Storage | Nephrin antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | Predicted: 135 kD + 10 N-linked glycosylation sites Observed: 200 kD |
| Gene Name | NPHS1 nephrin |
| Database Link | |
| Background | Nephrin Antibody: Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomal-recessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. |
| Synonyms | CNF; nephrin; NPHN |
| Note | Optimal dilutions for each application to be determined by the researcher. |
| Reference Data | |
| Protein Categories | Intracellular Proteins, Membrane Proteins |
| Protein Families | Druggable Genome, Transmembrane |
Reviews
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Citations
Elevated Prorenin Induces Podocyte Injury and Glomerular Fibrosis in cyp1a1-Prorenin Transgenic Rats
Gu, Liu, Wu et al
Int J Mol Sci (2026) 27 (17), 7888-7888 DOI: 10.3390/ijms27177888
Gu, Liu, Wu et al
Int J Mol Sci (2026) 27 (17), 7888-7888 DOI: 10.3390/ijms27177888
AI Summary: In this study, OriGene products—specifically the nephrin antibody (TA306037) and B7-1 antibody (TA303430)—were used to assess podocyte injury and protein expression changes in cyp1a1-prorenin transgenic rats, helping to elucidate the mechanisms by which elevated prorenin induces podocyte damage and glomerular fibrosis.
Snippet: embranes (Millipore, Burlington, MA, USA). Membranes were probed with antibodies against prorenin/renin, podocin (catalog No. sc-22298, Santa Cruz Biotechnology, Inc.), nephrin (catalog No. TA306037, OriGene Technologies, Inc., Rockville, MD, USA), B7-1 (catalog No. TA303430, OriGene Technologies, Inc.), plasminogen activator inhibitor-1 (PAI-1) (catalog No. 612025, BD Biosciences, San Jose, CA, U
A PAI-1 mutant retards diabetic nephropathy in db/db mice through protecting podocytes
Zhang, Gu, Lawrence et al
Exp. Physiol. (2014)
Zhang, Gu, Lawrence et al
Exp. Physiol. (2014)
Cited by 20
AI Summary: In this study, OriGene’s rabbit monoclonal anti-B7-1/CD80 IgG antibody was used to detect B7-1 protein expression in podocytes, contributing to the analysis of podocyte injury and the protective effects of the PAI-1 mutant in diabetic nephropathy.
Snippet: Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and rabbit monoclonal anti-B7- 1/CD80 IgG (OriGene Technologies, Inc., Rockville, MD, USA). The immunostaining ... obtained from OriGene Technologies and Invitrogen respectively. All experiments ... Cite SaveSaving
Targeting reduction of proteinuria in glomerulonephritis: Maximizing the antifibrotic effect of valsartan by protecting podocytes
Gu, Zhou, Noble et al
J Renin Angiotensin Aldosterone Syst (2012)
Gu, Zhou, Noble et al
J Renin Angiotensin Aldosterone Syst (2012)
Cited by 21
AI Summary: OriGene products, specifically the monoclonal rabbit anti-B7-1/CD80 IgG antibody, were used in this study to assess podocyte injury by detecting B7-1 expression levels in kidney tissue.
Snippet: However, large, long-term trials with hard outcomes are currently lacking for this potentially promising form of therapy. ... levels of nephrin and B7-1 were developed using polyclonal rabbit anti-nephrin IgG and monoclonal rabbit anti-B7-1/CD80 IgG (OriGene Technologies Inc
Showing 1-3 of 3 papers.
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