PRPF31 Goat Polyclonal Antibody
Specifications
| Product Data | |
| Application | WB |
|---|---|
| Recommended Dilution | ELISA: 1:128,000. WB: 1-3µg/ml. |
| Reactivity | Cow), Dog, Human (Expected from sequence similarity: Mouse, Pig, Rat |
| Antibody Host | Goat |
| Isotype | IgG |
| Clonality | Polyclonal |
| Immunogen | Peptide with sequence KELGNSLDKCKNNEN, from the internal region of the protein sequence according to NP_056444.2. |
| Buffer | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Concentration | lot specific |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Conjugation | Unconjugated |
| Storage | Store at -20°C as received. |
| Stability | Stable for 12 months from date of receipt. |
| Shipping | Blue Ice |
| Predicted Protein Size | 59070 Da |
| Gene Name | pre-mRNA processing factor 31 |
| Database Link | |
| Background | This gene encodes a component of the spliceosome complex and is one of several retinitis pigmentosa-causing genes. When the gene product is added to the spliceosome complex, activation occurs. |
| Synonyms | NY-BR-99; PRP31; RP11; SNRNP61 |
| Reference Data | |
| Protein Categories | Intracellular Proteins, Nervous system Diseases |
| Protein Families | Druggable Genome |
| Protein Pathways | Spliceosome |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Citations
Mesoporous Silica-Based Nanoparticles as Non-Viral Gene Delivery Platform for Treating Retinitis Pigmentosa
Valdés-Sánchez, Borrego-González, Montero-Sánchez et al
J Clin Med (2022) 11 (8) DOI: 10.3390/jcm11082170
Valdés-Sánchez, Borrego-González, Montero-Sánchez et al
J Clin Med (2022) 11 (8) DOI: 10.3390/jcm11082170
Cited by 23
AI Summary: OriGene’s anti-PRPF31 antibody (TA302582) was used in immunofluorescence experiments to detect transgene expression in retinal tissue, supporting the evaluation of gene delivery efficiency in this study.
Snippet: uorescence experiments were performed on eyecup sections obtained from wild-type mice. The retinal sections were incubated overnight at 4 °C with the primary antibodies anti-PRPF31 (1:100;TA302582, OriGene Technologies Inc., Rockville, MD, USA) and anti-GFP (1:200;Cell Signaling 2956). After incubation, samples were washed 3 times in PBS 0.2% Tween 20 (PBS-T) and incubated with appropriate Alex
Retinal pigment epithelium degeneration caused by aggregation of PRPF31 and the role of HSP70 family of proteins
Valdés-Sánchez, Calado, de la Cerda et al
Mol. Med. (2019) 26 (1), 1 DOI: 10.1186/s10020-019-0124-z
Valdés-Sánchez, Calado, de la Cerda et al
Mol. Med. (2019) 26 (1), 1 DOI: 10.1186/s10020-019-0124-z
Cited by 46
AI Summary: OriGene’s goat anti-PRPF31 antibody (TA302582) was used to detect and analyze PRPF31 protein localization and aggregation in retinal pigment epithelium cells, contributing to the investigation of disease mechanisms in this study.
Snippet: Samples were then washed in 0.2% Triton X-100/PBS (PBS-T) and blocked in 1% BSA/PBS-T at room temperature for 1 h. Incubation with the primary antibody goat anti-PRPF31 (1:100;OriGene Technologies Inc., Maryland, USA, TA302582) and mouse anti-Rhodopsin (1
Showing 1-2 of 2 papers.
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