CD4 Mouse Monoclonal Antibody [Clone ID: vpg34]

Name: CD4 Mouse Monoclonal Antibody [Clone ID: vpg34]
Brand: OEM
SKU: SM588F
Price: 980 USD
Availability: InStock

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SKU

SM588F

CD4 mouse monoclonal antibody, clone vpg34, FITC

Datasheet SDS

<strong>Published customer image:</strong> <br>FITC conjugated Mouse anti Feline CD4 antibody, clone vpg34 (<strong>SM588F</strong>) used for the identification of CD4<sup>+</sup> lymphocytes in peripheral blood smears by immunocytochemistry.<br><strong>Image caption:</strong><br>Lymphocyte subsets after <em>M. haemofelis</em> exposure in the ten SPF cats. The five cats in group A had undergone previous “<em>Cand. M. turicensis</em>” infection and the five cats in group B were naïve control cats. The <em>M. haemofelis</em> exposure took place on day 0. A) T cells (CD5<sup>+</sup> MHCII<sup>+</sup>) counts, B) CD4<sup>+</sup> T cell counts, C) CD8<sup>+</sup> T cell counts, D) CD4<sup>+</sup>/CD8<sup>+</sup> ratio, E) CD4<sup>+</sup>CD25<sup>+</sup> activated T cells counts and F) B cell (CD45R/B220<sup>+</sup>) counts. No significant differences between groups A and B were observed on day 0; significant differences between the two groups observed thereafter are indicated with an asterisk (pMWU <0.05). Significant differences over time (pF<0.05 and pD<0.05) and the corresponding significantly decreased or increased values are indicated with an arrow (black solid for group A and gray open for group B). In detail, increased T cell counts were observed in group A on days 2, 6 and 62 compared with day 83 and in group B on day 9 and 20 compared with day 83 (A). Decreased CD8<sup>+</sup> T lymphocyte counts were observed in group A on day 20 compared with days 57 and 83 (C). In the CD4<sup>+</sup>/CD8<sup>+</sup> ratio, in group A, significantly lower values were observed on day 48 compared to days 0 and 41, whereas in group B, higher counts were observed on day 0 compared to days 9 and 83 and on and 41 compared to day 9 (D). CD4<sup>+</sup>CD25<sup>+</sup> cell counts varied significantly over time in cats in group B, with lower values on days 2, 9 and 27 compared with day 6 (E). B cells varied significantly over time in cats in group A, with transiently decreased values on days 20, 27, 34 and 41 compared with days 9, 62, 76 and 83 (F). No values were available for CD4<sup>+</sup>CD25<sup>+</sup> on day 83.<br><br>From: Baumann J, Novacco M, Willi B, Riond B, Meli ML, Boretti FS, Hofmann-Lehmann R.<br>Lack of cross-protection against <em>Mycoplasma haemofelis</em> infection and signs of enhancement in "<em>Candidatus Mycoplasma turicensis</em>"-recovered cats.<br><strong><a href="https://www.ncbi.nlm.nih.gov/pubmed/26403079"target="_blank">Vet Res. 2015 Sep 24;46:104</a></strong>.<br>This is from an open access article distributed under the terms of the <a href="https://creativecommons.org/licenses/by/4.0/"target="_blank">Creative Commons Attribution License</a>.

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Specifications

Specifications
Product Data
Clone Name	vpg34
Application	FC
Recommended Dilution	Flow Cytometry: Neat - 1/10; Use 10µl of the suggested working dilution to label 10e6 lymphocytes in 100µl.
Reactivity	Feline
Antibody Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Immunoaffinity purified feline CD4. Spleen cells from immunised BALB/c were fused with cells of the NSO mouse myeloma cell line.
Specificity	This antibody recognises the homologue of the human CD4 antigen.
Buffer	Containing 0.09% Sodium Azide and 1% Bovine Serum Albumin Label: FITC State: Liquid purified IgG Label: Fluorescein Isothiocyanate Isomer 1
Concentration	lot specific
Conjugation	FITC
Storage	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
Stability	Shelf life: one year from despatch.
Shipping	Blue Ice
Database Link	P79355
Background	CD4 is not expressed on feline monocytes.
Synonyms	T-cell surface antigen T4/Leu-3
Reference Data

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Documents

Product Manuals
Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
SDS for SM588F

Resources

Antibody Resources
Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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CD4 Mouse Monoclonal Antibody [Clone ID: vpg34]

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CD4 Mouse Monoclonal Antibody [Clone ID: vpg34]

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