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Horseradish Peroxidase / HRP (Grade I) Mouse Monoclonal Antibody [Clone ID: HP-03]

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SKU
SM3056P
Horseradish Peroxidase / HRP (Grade I) mouse monoclonal antibody, clone HP-03, Purified
Size:
100 ug
Conjugation:
Unconjugated
  $280.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name HP-03
Application ELISA, IF, WB
Recommended Dilution ELISA.
Western blot.
Immunocytochemistry.
The antibody was successfully used for staining of formaldehyde-fixed, Triton X permeabilized cells transfected with HRP gene.
Reactivity Horseradish Peroxidase, Human
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Horseradish peroxidase (Grade I)
Specificity The antibody reacts with Horseradish Peroxidase.
It is suitable for PAP detection system.
Buffer PBS, pH~7.4 with 15 mM Sodium Azide as preservative
State: Purified
State: Liquid purified Ig fraction (> 95 pure by SDS-PAGE)
Concentration lot specific
Purification Purified by protein-A affinity chromatography.
Conjugation Unconjugated
Storage Store undiluted at 2-8°C.
DO NOT FREEZE!
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
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Documents
Resources

file-alt Citations

Phosphorylation of Androgen Receptor by mTORC1 Promotes Liver Steatosis and Tumorigenesis
Ren, Zhang, Sun et al
Hepatology (2021)
Cited by 34
AI Summary: OriGene products, specifically the EDTA Buffer pH=8.0 (ZLI-9067) for antigen retrieval and the antibody diluent buffer (ZLI-9029) for antibody incubation, were used to facilitate immunohistochemical analyses in the study investigating androgen receptor phosphorylation in liver steatosis and tumorigenesis.
Snippet: The Antigen retrieval was conducted by pressure cooker at full pressure for 3min in the EDTA Buffer pH=8.0 (ORIGENE # ZLI-9067) and then blocked in 10% FBS for 1 hour antibodies diluted in antibodies diluent buffer (ORIGENE # ZLI-9029) at 4℃ overnight and with
IHC
Mitochondrial pathway of the lysine demethylase 5C inhibitor CPI-455 in the Eca-109 esophageal squamous cell carcinoma cell line
Xue, Li, Yu
World J Gastroenterol (2021) 27 (16), 1805-1815 DOI: 10.3748/wjg.v27.i16.1805
Cited by 6
AI Summary: In this study, OriGene's HRP-labeled mouse secondary antibody was used for western blot detection to analyze protein expression changes in Eca-109 cells treated with the KDM5C inhibitor CPI-455.
Snippet: and GAPDH monoclonal antibodies (1:1000;Santa Cruz Biotechnology, Inc., United States) were added and the membranes were incubated overnight at 4 °C and then with horseradish peroxidase (HRP)-labeled mouse secondary antibody (1:3,000;Origene
FigureFigureFigure
WB FC
Osteopontin promotes cancer cell drug resistance, invasion, and lactate production and is associated with poor outcome of patients with advanced non-small-cell lung cancer
Ouyang, Huang, Jin et al
Onco Targets Ther (2018) 11, 5933-5941 DOI: 10.2147/OTT.S164007
AI Summary: OriGene's HRP/fragment antigen binding polymer-conjugated secondary antibody was used for immunostaining to detect osteopontin expression in NSCLC tumor samples, contributing to the study's analysis of OPN's role in cancer progression and drug resistance.
Snippet: were immunostained with 2 µg/mL anti-OPN primary antibody (Bioworld Technology Co, Ltd, Nanjing, China) overnight at 4°C and stained with a horseradish peroxidase (HRP)/fragment antigen binding polymer-conjugated secondary antibody (OriGene Technologies
FigureFigureFigure
IHC
Showing 1-3 of 3 papers.
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