HIV-1 + HIV-2 Protease Mouse Monoclonal Antibody [Clone ID: 1696]

SKU
SM3051AF
HIV-1 + HIV-2 Protease mouse monoclonal antibody, clone 1696, Azide Free
  $325.00
2 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Clone Name 1696
Application ELISA, FN, WB
Recommended Dilution ELISA.
Western blotting: 0.5 μg/ml (Reducing conditions).
Sample Preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with reducing Laemmli SDS-PAGE sample buffer.
Functional Assay: Antibody SM3051A strongly inhibits the enzyme activity of HIV-1 and HIV-2 proteases.
Reactivity Human Immunodeficiency Virus 1, Human Immunodeficiency Virus 2
Antibody Host Mouse
Isotype IgG1
Clonality Monoclonal
Immunogen Bacterially expressed full-length HIV-1 Protease
Specificity The antibody recognizes free N-terminus of mature HIV protease HIV1 and HIV2, but it does not react with the precursor.
Buffer Azide Free Phosphate Buffered saline (PBS), pH~7.4, 0.2 μm filter sterilized
State: Azide Free
State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE)
Concentration lot specific
Purification Affinity Chromatography on Protein A
Conjugation Unconjugated
Storage

Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.

Stability Shelf life: one year from despatch.

Shipping Blue Ice
Background The HIV protease (PR) hydrolyzes polyproteins of HIV virus into functional protein products that are essential for its assembly and subsequent activity. This maturation process occurs as the virion buds from the host cell. HIV protease inhibitors are used in the treatment of patients with AIDS and were considered the first breakthrough in over a decade of AIDS research. HIV protease inhibitors can lower the viral load carried by AIDS patents.
Synonyms HIV1, HIV-I, Human immunodeficiency virus type 1
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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