Cd8b Mouse Monoclonal Antibody [Clone ID: 341]
Specifications
| Product Data | |
| Clone Name | 341 |
|---|---|
| Application | FC, IP, WB |
| Recommended Dilution | Flow Cytometry: 1/100 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunoprecipitation. |
| Reactivity | Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Rat/mouse T cell hybrids expressing CD8. Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63Ag8.653 myeloma cell line. |
| Specificity | This antibody recognises the beta chain of the rat CD8 cell surface antigen. This antibody is reported to block proliferative and cytotoxic responses of CD8 +ve effector cells in vitro. |
| Buffer | PBS, pH7.4 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG |
| Concentration | lot specific |
| Purification | Affinity chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD8b molecule |
| Database Link | |
| Background | CD8 is expressed on thymocytes and peripheral cytotoxic/suppressor cells. CD8b is not expressed by NK cells or by intestinal intraepithelial T cells. |
| Synonyms | CD8B, CD8B1 |
| Reference Data | |
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