Cd8a Mouse Monoclonal Antibody [Clone ID: OX-8]
Specifications
| Product Data | |
| Clone Name | OX-8 |
|---|---|
| Application | FC, IF, IHC, IP, WB |
| Recommended Dilution | Flow Cytometry: 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin Sections: This product does not require protein digestion pre-treatment of paraffin embedded sections; This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Western blot. Immunofluorescence. Immunoprecipitation. |
| Reactivity | Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. |
| Specificity | This antibody Mouse anti Rat CD8α, clone MRC OX-8, recognizes the rat CD8 alpha cell surface antigen, expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. Clone MRC OX-8 is suitable for use in in vitro blocking studies (Popov et al.2001). Mouse anti Rat CD8α, clone MRC OX-8 has been described reacting with paraffin-embedded material following PLP Fixation (periodate-lysine paraformaldehyde) (Whiteland et al. 1995). Mouse anti Rat CD8α, clone MRC OX-8 is routinely tested in flow cytometry on rat splenocytes. |
| Buffer | PBS, pH 7.2 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG |
| Concentration | lot specific |
| Purification | Affinity chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD8a molecule |
| Database Link | |
| Background | The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. |
| Synonyms | CD8 alpha chain, CD8A, MAL |
| Reference Data | |
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