Cd8a Mouse Monoclonal Antibody [Clone ID: OX-8]
Specifications
| Product Data | |
| Clone Name | OX-8 |
|---|---|
| Application | FC, IF, IHC, IP, WB |
| Recommended Dilution | Western Blot. Immunoprecipitation. Immunofluorescence. Flow Cytometry: 1/50-1/100. Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin sections: This product does not require protein digestion pre-treatment of paraffin embedded sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections. |
| Reactivity | Rat |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Rat thymocyte membrane glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. |
| Specificity | This antibody recognises the CD8 alpha cell surface antigen. Clone MRC OX-8 is suitable for use in in vitro blocking studies. We recommend the use of SM259A for this purpose. This clone has been described reacting with paraffin-embedded material following PLP Fixation (periodatelysine paraformaldehyde). |
| Buffer | PBS, pH 7.2 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD8a molecule |
| Database Link | |
| Background | CD8 is expressed by a subset of T lymphocytes, most thymocytes and the majority of NK cells. |
| Synonyms | CD8 alpha chain, CD8A, MAL |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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