CD4 Mouse Monoclonal Antibody [Clone ID: 44.38]
Specifications
| Product Data | |
| Clone Name | 44.38 |
|---|---|
| Application | FC, IF, IHC, IP |
| Recommended Dilution | Immunoprecipiation. Immunocytochemistry. Immunohistochemistry on Frozen Sections. Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 106 cells in 100 µl. |
| Reactivity | Goat, Sheep |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Fetal thymocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse P3-NS1/1-Ag-4-1 myeloma cell line. |
| Specificity | This antibody (Clone 44.38) recognizes the ovine CD4 cell surface glycoprotein, which is expressed by a subset of mature T lymphocytes. This clone will immunoprecipitate a 56kD band under Reducing Conditions. |
| Buffer | PBS State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein G |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Database Link | |
| Background | CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase. |
| Synonyms | T-cell surface antigen T4/Leu-3 |
| Reference Data | |
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