CD177 Mouse Monoclonal Antibody [Clone ID: MEM-166]
Product Data | |
Clone Name | MEM-166 |
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Application | FC, IP, WB |
Recommended Dilution | Western Blot (Non-Reducing Conditions). Immunoprecipitation. Flow Cytometry: 1 µg/ml. |
Reactivity | Human, Primate |
Antibody Host | Mouse |
Isotype | IgG1 |
Clonality | Monoclonal |
Immunogen | Human Granulocytes |
Specificity | This antibody reacts with CD177 (Neutrophil specific antigen 1), a 60 kDa GPI-linked cell surface glycoprotein of uPAR family, expressed on granulocytes and in bone marrow early erythroblasts, megakaryocytes, promyelocytes and myelocytes. |
Buffer | PBS State: Purified State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE) Preservative: 15 mM Sodium Azide |
Concentration | lot specific |
Purification | Affinity Chromatography on Protein A |
Conjugation | Unconjugated |
Storage | Store undiluted at 2-8°C. DO NOT FREEZE! |
Stability | Shelf life: one year from despatch. |
Shipping | Blue Ice |
Gene Name | CD177 molecule |
Database Link | |
Background | CD177 (NP1), a glycosyl phosphatidylinositol (GPI) linked N glycosylated cell surface glycoprotein, was first described in a case of neonatal alloimmune neutropenia and analyses showed cell surface expression of a 60 kDa protein, 14 kDa greater than the predicted size, probably due to the presence of 3 potential N glycosylation sites. Immunohistochemistry demonstrated expression in bone marrow early erythroblasts, megakaryocytes, promyelocytes, and myelocytes. The neutrophil NB1 antigen (CD177) is expressed by 97% of the caucasian population. |
Synonyms | NB1, PRV1, HNA-2a, HNA2a |
Reference Data |
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