Feline Coronavirus Mouse Monoclonal Antibody [Clone ID: FIPV3-70]
$870.00
5 Days*
Specifications
| Product Data | |
| Clone Name | FIPV3-70 |
|---|---|
| Application | ELISA, FC, IF, IHC, WB |
| Recommended Dilution | Flow Cytometry. Immunofluorescence. ELISA: This antibody can be used as a Capture reagent in ELISA assays in combination with AM01094BT-N as a detection reagent. Western Blot: Western blotting, using this antibody under reducing conditions, results in a specific band of 50-56 kDa that represents the nucleocapsid. Immunohistochemistry on Frozen and Paraffin Sections: Microwave induced antigen retrieval in 10 mM citrate buffer is recommended prior to staining of paraffin embedded sections. |
| Reactivity | Bovine Coronavirus, Canine Coronavirus, Feline Coronavirus, Feline Infectious Peritonitis Virus, Transmissable Gastroenteritis Virus |
| Antibody Host | Mouse |
| Isotype | IgG2a |
| Clonality | Monoclonal |
| Immunogen | Coronavirus cocktail. |
| Specificity | This antibody reacts with Feline infectious peritonitis virus (FIPV) type 1 and 2 and is known to be specific for the nucleocapsid. It is also known to react with canine coronavirus (CCV), pig coronavirus transmissable gastroenteritis virus (TGEV) and ferret coronavirus. Some specific activity has been detected against bovine coronavirus (BCV). Clone FIPV3-70 exhibits negative reactivity against Feline Leukemia virus, Feline Immunodeficiency virus, Feline Calcivirus, Feline Herpes virus, Canine Adenovirus (type 2), Canine Distemper virus, Canine Parvovirus and Canine Parainfluenza virus. |
| Buffer | PBS, pH 7.2 containing 0.05% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction. |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein A. |
| Conjugation | Unconjugated |
| Storage | Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Reference Data | |
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Citations
A novel compound targets the feline infectious peritonitis virus nucleocapsid protein and inhibits viral replication in cell culture
Mohseni, Royster, Ren et al
J Biol Chem (2023) 299 (3), 102976 DOI: 10.1016/j.jbc.2023.102976
Mohseni, Royster, Ren et al
J Biol Chem (2023) 299 (3), 102976 DOI: 10.1016/j.jbc.2023.102976
Cited by 4
AI Summary: OriGene’s Anti-FIP N mouse monoclonal antibody (Cat # SM1870P) was used in this study to detect the feline coronavirus nucleocapsid protein, enabling the assessment of viral protein expression and the evaluation of the novel compound K31’s antiviral effects.
Snippet: 's modified Eagle's medium (DMEM) containing 10% fetal bovine serum (FBS) and penicillin–streptomycin (100 μg/ml) in a CO2 incubator. Anti-FIP N mouse monoclonal antibody (Cat # SM1870P) was from Origene. Anti-guineapig (Cat #: 926-68077) and anti-mouse (Cat #: 926-68072) secondary antibodies were from LI-COR. The PCR reagents were from GenScript. The reagents for real-time PCR were from Appli
Polyarteritis Nodosa in a Cat with Involvement of the Central and Peripheral Nervous Systems
Salvadori, Vezzosi, Marchetti et al
J Comp Pathol (2019) 167, 6-11 DOI: 10.1016/j.jcpa.2018.11.003
Salvadori, Vezzosi, Marchetti et al
J Comp Pathol (2019) 167, 6-11 DOI: 10.1016/j.jcpa.2018.11.003
Cited by 2
Showing 1-2 of 2 papers.
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