Endothelial Cells (RECA-1 antigen) Mouse Monoclonal Antibody [Clone ID: HIS52]

Name: Endothelial Cells (RECA-1 antigen) Mouse Monoclonal Antibody [Clone ID: HIS52]
Brand: OEM
SKU: SM1837PS
Price: 490 USD
Availability: InStock

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SKU

SM1837PS

Endothelial Cells (RECA-1 antigen) mouse monoclonal antibody, clone HIS52, Purified

Datasheet SDS

<strong>Published customer image:</strong> <br>Mouse anti Rat RECA-1 antibody, clone HIS52 (<strong>MCA970R</strong>) used for the detection of RECA-1 expressing cells by immunofluorescence.<br><strong>Image caption</strong><br>Transduction of AdV-eGFP/AdV-ZFP-VEGF into the spinal cord. (A) Photomicrographs showing a transverse section of rat spinal cord obtained adjacent to the injury site 10 days after spinal cord injury and AdV-eGFP injection. eGFP signal was detected in both the gray matter and white matter. (B) High-power (63X) confocal images show that the AdV-eGFP vector (green) transfected neurons (NeuN), astrocytes (GFAP), oligodendrocytes (CC1) and endothelial cells (RECA-1). Cells have been counter-stained with DAPI (blue) as nuclear marker. (C) Bar graph displays quantification of transduced cell types ± SEM, as identified by the cell-specific markers NeuN, GFAP, RECA-1 and CC1. (D) Evaluation of AdV-ZFP-VEGF gene transfer. Western blot showed that the NFκB p65 rabbit polyclonal antibody recognizes the p65 activation domain in the AdV-ZFP-VEGF treated animals. The higher molecular weight bands are endogenous NFκBp65 fragments, which are also recognized by the antibody; however, these bands are present in both the control and treatment groups. The lower band (arrow) corresponds to the AdV-ZFP-VEGF and was only present in the treated animals. Lower panel shows actin expression as a protein control. Scale bar: 1000 μm for A; 100 μm for B.<br><br>From: Figley SA, Liu Y, Karadimas SK, Satkunendrarajah K, Fettes P, <em>et al</em>. <br>Delayed Administration of a Bio-Engineered Zinc-Finger VEGF-A Gene Therapy Is Neuroprotective and Attenuates Allodynia Following Traumatic Spinal Cord Injury.<br><strong><a href="https://www.ncbi.nlm.nih.gov/pubmed/24846143"target="_blank" rel="noopener">PLoS ONE (2014) 9(5): e96137</a></strong>.<br>doi: 10.1371/journal.pone.0096137.<br>This image is from an open access article distributed under terms of a <a href="https://creativecommons.org/licenses/by/4.0/"target="_blank" rel="noopener">Creative Commons Attribution License</a>.

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Specifications

Specifications
Product Data
Clone Name	HIS52
Application	IF, IHC
Recommended Dilution	Immunofluorescence. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: clone HIS52 has been reported by customers to work on Formalin/PFA-fixed paraffin-embedded sections.
Reactivity	Rat
Antibody Host	Mouse
Isotype	IgG1
Clonality	Monoclonal
Immunogen	Stromal cells from rat lymph node.
Specificity	SM1837PS recognises RECA-1, a cell surface antigen which is expressed by all rat endothelial cells. Does not react with Mouse, Guinea Pig, Rabbit, Pig, Goat, Sheep or Chicken.
Buffer	PBS with 0.09% Sodium Azide as preservative State: Purified State: Liquid purified IgG fraction
Concentration	lot specific
Purification	Protein G Affinity Chromatography
Conjugation	Unconjugated
Storage	Store the antibody undiluted at 2-8°C for up to one month or at -20°C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Stability	Shelf life: one year from despatch.
Shipping	Blue Ice
Synonyms	Endothelial Cell marker
Reference Data

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Documents

Product Manuals
Goat anti-Mouse IgG [H&L] Western Blotting Luminol Kit
FAQs
Antibody and Western Standard
SDS
SDS for SM1837PS

Resources

Antibody Resources
Product Overview Video: TrueMAB Video Tutorial: Video Tutorial: Antibody specificity and Knockout(KO) lysates Western Blot Protocol IHC Protocol IF Protocol FACS Protocol Immunoprecipitation (IP) Protocol IP protocol with magnetic beads

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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Endothelial Cells (RECA-1 antigen) Mouse Monoclonal Antibody [Clone ID: HIS52]

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Endothelial Cells (RECA-1 antigen) Mouse Monoclonal Antibody [Clone ID: HIS52]

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