HLAB (HLA-B) Mouse Monoclonal Antibody [Clone ID: BB7.1]

CAT#: SM1304F

HLAB (HLA-B) mouse monoclonal antibody, clone BB7.1, FITC

Product Images

Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN HLAB7-FITC

Specifications

Product Data

• Clone Name: BB7.1
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 1x10⁶ cells in 100 µl whole blood.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Papain solubilized HLA-B7 antigen.
• Specificity: This antibody recognises the Human HLA-B7 antigen and does not cross-react with HLA-B27 or other related HLA antigens.
  It can be used to distinguish true HLA-B27 positives from false HLA-B27 positives (i.e. HLA-B7 positive) in the investigation of such diseases as ankylosing spondylitis and anterior uveitis.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Should this product contain a precipitate we recommend micro-centrifugation before use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: major histocompatibility complex, class I, B
• Database Link:
  Entrez Gene 3106 Human
  UniProt ID P01889
• Background: HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described.
  The HLA B gene is part of the human HLA complex on chromosome 6 and there are a large number of variant alleles of this gene.
• Synonyms: HLAB, MHC class I antigen B7