B7-2 (CD86) Mouse Monoclonal Antibody [Clone ID: BU63]
$255.00
5 Days*
Specifications
| Product Data | |
| Clone Name | BU63 |
|---|---|
| Application | FC, IHC, IP |
| Recommended Dilution | Immunoprecipitation. Flow Cytometry: 1/20-1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunohistochemistry on Frozen Sections: 1/20-1/100. Recommended Positive Control: Tonsil. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Human peripheral blood lymphocytes. Spleen cells from immunised mice were fused with cells of the P3 X63 Ag8.653 myeloma cell line. |
| Specificity | This antibody recognises CD86. Domain depletion epitope mapping studies indicate that the binding site of Mouse anti Human CD86, Clone Bu63 is located within the Ig-v-like domain of Human CD86 (Jeanin et al. 1997). |
| Buffer | 0.1M Tris buffered saline, pH 8.2 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein A |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | CD86 molecule |
| Database Link | |
| Background | CD86 is a 70kD cell surface protein expressed by activated B cells and monocytes. CD86 is also known as B7-2 and is the second ligand for CD28 and CTLA-4. It may have an important role in costimulation of T cells in the primary immune response. |
| Synonyms | CD28LG2, Activation B7-2 antigen, B70, BU63, B7.2, FUN-1 |
| Reference Data | |
| Protein Categories | CD Markers, Intracellular Proteins, Membrane Proteins |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.